Issue 25, 2022, Issue in Progress

Enhanced detection sensitivity through enzyme-induced precipitate accumulation in LSPR-active nano-valleys

Abstract

Biomolecule detection based on the localized surface plasmon resonance (LSPR) phenomenon has advantages in label-free detection, good sensitivity, and measurement simplicity and reproducibility. However, in order to ultimately be used for actual diagnosis, the ability to detect trace amounts of biomarkers is necessary, which requires the development of signal enhancement strategies that enable ultrasensitive detection. In this paper, we provide a straightforward and efficient route to boost LSPR sensitivity based on multiple sample washings. We found that repeated washing and drying cycles lead to a shift in the LSPR peak in a concentration-dependent manner, where this process drives the accumulation of a precipitate, formed by an enzyme reaction with target specificity, in the sample's LSPR active plasmonic nano-valley structure. Results show that the washing and drying process leads to a signal enhancement of more 200 times compared to a sensor with only enzyme-based amplification. To maximize this effect, optimization of the plasmonic nanostructure was also carried out to finally achieve atto-molar detection of miRNA with a distinguishable LSPR peak shift.

Graphical abstract: Enhanced detection sensitivity through enzyme-induced precipitate accumulation in LSPR-active nano-valleys

Supplementary files

Article information

Article type
Paper
Submitted
28 Feb 2022
Accepted
11 May 2022
First published
23 May 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 15652-15657

Enhanced detection sensitivity through enzyme-induced precipitate accumulation in LSPR-active nano-valleys

S. Kwak, J. Wi, J. Lee, C. Kim and H. Na, RSC Adv., 2022, 12, 15652 DOI: 10.1039/D2RA01331B

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