Issue 9, 2022

A magneto-controlled biocatalytic cascade with a fluorescent output

Abstract

A biocatalytic cascade based on concerted operation of pyruvate kinase and luciferase with a bioluminescent output was switched reversibly between low and high activity by applying an external magnetic field at different positions or removing it. The enzymes participating in the reaction cascade were bound to magnetic nanoparticles to allow their translocation or aggregation/dispersion to be controlled by the magnetic field. The reaction intensity, measured as the bioluminescent output, was dependent on the effective distances between the enzymes transported on the magnetic nanoparticles controlled by the magnets.

Graphical abstract: A magneto-controlled biocatalytic cascade with a fluorescent output

Supplementary files

Article information

Article type
Communication
Submitted
25 Nov 2021
Accepted
02 Feb 2022
First published
03 Feb 2022

Org. Biomol. Chem., 2022,20, 1869-1873

Author version available

A magneto-controlled biocatalytic cascade with a fluorescent output

A. Othman, O. Smutok, Y. Kim, S. Minko, A. Melman and E. Katz, Org. Biomol. Chem., 2022, 20, 1869 DOI: 10.1039/D1OB02313F

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