Dual enzyme co-immobilization on reversibly soluble polymers for the one-pot conversion of ferulic acid from wheat bran

Abstract

The difficulty of using immobilized enzyme to decompose wheat bran to produce ferulic acid lies in the recovery of enzyme from solid-rich wheat bran hydrolysates. In this study, two enzymes (feruloyl esterase and xylanase) were successfully immobilized on the reversible soluble vector Eudragit L-100. The co-immobilized enzymes were used to decompose wheat bran to produce ferulic acid through the determination of enzymatic properties and catalytic conditions. The yield of ferulic acid reached 35% of the total ferulic acid content in wheat bran under these conditions. The reversible and soluble nature of the carrier made it simpler to recover co-immobilized enzymes from solid matrices. The co-immobilized enzymes could be reused for 5 cycles with more than 60% of the activity left, which verified the feasibility of using reversible soluble carrier to immobilize ferulic esterase and xylanase.