Preparation of carbon-based metal organic framework-modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

Abstract

In this study, a carbon-based Cu²⁺-immobilized metal–organic framework-modified molecularly imprinted polymer (C@GI@Cu-MOFs@MIPs) adsorbent was prepared using bovine hemoglobin (BHb) as a template molecule with carbon spheres as carriers for the selective separation and enrichment of BHb. This paper presents a systematic investigation of the influence of sample concentration, sample pH, ionic strength, and eluent type on the adsorption performance of the prepared adsorbent. It was experimentally proven that the adsorbent could be reused even after six adsorption–desorption cycles without significant deterioration in the activity. Moreover, the C@GI@Cu-MOFs@MIPs have good binding behavior toward BHb with fast binding kinetics (45 min), high adsorption capacity (1051.1 mg g⁻¹), and satisfactory selectivity in the presence of interfering proteins. In addition, C@GI@Cu-MOFs@MIPs were successfully applied to selectively identify, separate, and enrich BHb from real bovine blood samples as confirmed by SDS-PAGE, and thus are expected to be used in the pretreatment of proteomic analysis.