Endogenous hydrogen peroxide can efficiently regulate CRISPR-Cas9 based gene editing†
Abstract
CRISPR-Cas9 genome editing technology is an emerging tool in life science, clinical and plant research. This technique offers a remarkable specificity and efficiency in gene editing. In this study, we demonstrated that hydrogen peroxide can regulate Cas9 by triggering boronated-lysine bond cleavage in vitro. This offers an alternative to UV light, which may induce undesirable genetic changes. Our technique is also an invaluable tool in genetic code expansion, particularly in studying enzymatic, cellular and other biological processes.