Issue 24, 2022

An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols

Abstract

Green, efficient, and sustainable flow synthesis of intermediate chiral aryl alcohols is critical for continuous drug-manufacturing. Enzyme immobilization endows flow biocatalysis with higher application potential; however, conventional methods are limited by cumbersome operations, a trade-off between activity and stability, and low loading due to the required support. Here, for the first time, we demonstrated the microfluidic induction of alcohol dehydrogenase to form enzyme-assembled gels (EAGs) in a microreactor, with controllable mechanical properties and a porous architecture. Six chiral aryl alcohols were successfully synthesized by this approach in flow asymmetric reduction. Moreover, the support-free EAG was capable of in situ flow immobilization and reversible de-immobilization without reactor disassembly. The EAG monolithic microreactor presented an excellent space–time yield of 88.05 g L−1 h−1 and a high total turnover number of 62 600 after 100 h of operation (over 1300 reactor volumes), highlighting its high activity and stability.

Graphical abstract: An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols

Supplementary files

Article information

Article type
Paper
Submitted
17 Aug 2022
Accepted
07 Oct 2022
First published
11 Oct 2022

Green Chem., 2022,24, 9508-9518

An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols

Q. Chen, Y. An, M. Feng, J. Li, Y. Li, F. Tong, G. Qu, Z. Sun, Y. Wang and G. Luo, Green Chem., 2022, 24, 9508 DOI: 10.1039/D2GC03082A

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