Molecular actions of different functional oligosaccharides on intestinal integrity, immune function and microbial community in weanling pigs

Abstract

This study investigated the effects of different functional oligosaccharides on the growth performance, intestinal barrier function and gut microbiota of weanling piglets. A total of 192 weanling piglets were randomly allocated into 4 dietary groups, which were a control (CON), CON + 0.1% xylo-oligosaccharide (XO), CON + 0.1% isomalto-oligosaccharide (IM) or CON + 0.08% mannan-oligosaccharide (MO) diet. Results showed that the XO and MO groups showed lower diarrhea incidence in weanling piglets compared with the CON group (P < 0.05). Colonic goblet cell numbers and the mRNA expression of mucin-2 (P < 0.05) were greater in all functional oligosaccharide groups. Both the XO and MO groups had a greater concentration of sIgA, IL-10, SOD and GSH-Px and higher antioxidase activity, and the XO group had a lower protein expression of NF-κB in the ilea of piglets (P < 0.05) compared with the CON group. The XO group had a greater concentration of butyrate and an abundance of Bifidobacterium in the ileum (P < 0.05). The MO group had a greater ileal concentration of acetate compared with the additional dietary treatments and greater butyrate compared with the IM and CON groups (P < 0.05). In summary, XO increased the growth of Bifidobacterium, butyrate production and intestinal antioxidant capacity; however, MO promoted the expression of tight junction proteins and the intestinal immune function to reduce piglet diarrhea.