Issue 8, 2023

Slice & Dice: nested spin–lattice relaxation measurements


Spin–lattice relaxation rate (R1) measurements are commonly used to characterize protein dynamics. However, the time needed to collect the data can be quite long due to long relaxation times of the low-gamma nuclei, especially in the solid state. We present a method to collect backbone heavy atom relaxation data by nesting the collection of datasets in the solid state. This method results in a factor of 2 to 2.5 times faster data acquisition for backbone R1 relaxation data for the 13C and 15N sites of proteins.

Graphical abstract: Slice & Dice: nested spin–lattice relaxation measurements

Supplementary files

Article information

Article type
28 Jul 2022
12 Oct 2022
First published
12 Oct 2022
This article is Open Access
Creative Commons BY license

Phys. Chem. Chem. Phys., 2023,25, 6044-6049

Slice & Dice: nested spin–lattice relaxation measurements

W. T. Franks, J. Tognetti and J. R. Lewandowski, Phys. Chem. Chem. Phys., 2023, 25, 6044 DOI: 10.1039/D2CP03458A

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