NAPT, an unbiased approach for sequential analysis of the protein N- and C-terminome

Abstract

Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT) for selective separation of protein termini. Altogether, we identified 2458 protein N-termini and 3056 protein C-termini from the HeLa cell line.