Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Abstract

Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.

Graphical abstract: Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Supplementary files

Article information

Article type
Communication
Submitted
03 Apr 2022
Accepted
12 May 2022
First published
14 May 2022

Chem. Commun., 2022, Advance Article

Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Y. Mei, Z. Liu, M. Liu, J. Gong, X. He, Q. Zhang and Y. Tian, Chem. Commun., 2022, Advance Article , DOI: 10.1039/D2CC01909D

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