Issue 40, 2022

Auto-cycling primer extension for amplified microRNA detection

Abstract

The current polymerase-based nucleic acid amplification techniques cannot maintain continuous polymerization reactions unless by changing the temperature or adding additional reagents (e.g. the second enzyme or betaine), which undoubtedly increases the cost and operation steps. Herein, a new isothermal nucleic acid amplification technique, termed auto-cycling primer extension (APE), is presented. It repeatedly extends short DNA primers to longer DNA hairpins, by combining a DNA-based copy-and-release hairpin (CRH) and palindromic sequence design. The experimental results showed that we could realize the amplification detection of miRNA by a reasonable probe design.

Graphical abstract: Auto-cycling primer extension for amplified microRNA detection

Supplementary files

Article information

Article type
Communication
Submitted
29 Mar 2022
Accepted
19 Apr 2022
First published
19 Apr 2022

Chem. Commun., 2022,58, 6020-6023

Auto-cycling primer extension for amplified microRNA detection

H. Wang, J. Wang, R. Liu, Z. Liu, K. Wang and J. Huang, Chem. Commun., 2022, 58, 6020 DOI: 10.1039/D2CC01784A

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