Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA

Yoshiya Kikukawa; Ryoji Kawazoe; Ryo Miyahara; Takato Sakurada; Yusuke Nagata; Shigeki Sasaki; Yosuke Taniguchi

doi:10.1039/D2CC01372J

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Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA†

Yoshiya Kikukawa,^a Ryoji Kawazoe,^a Ryo Miyahara,^a Takato Sakurada,^a Yusuke Nagata,^a Shigeki Sasaki

^b and Yosuke Taniguchi

*^a

Author affiliations

* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka, Japan
E-mail: taniguch@phar.kyushu-u.ac.jp

^b Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch Machi, Sasebo, Japan

Abstract

The identification of the position of 8-oxo-2′-deoxyguanosine (8-oxo-dG) in DNA is important to clarify the pathogenesis of many diseases. We herein developed a purine-1,3-diazaphenoxazine triphosphate (dPdapTP) and described the first example of detecting the presence of 8-oxo-dG by amplifying it several hundred times after the multiple-turnover single nucleotide primer extension reactions.

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Article information

DOI: https://doi.org/10.1039/D2CC01372J
Article type: Communication
Submitted: 08 Mar 2022
Accepted: 06 Apr 2022
First published: 08 Apr 2022

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Chem. Commun., 2022,58, 5399-5402

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Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA

Y. Kikukawa, R. Kawazoe, R. Miyahara, T. Sakurada, Y. Nagata, S. Sasaki and Y. Taniguchi, Chem. Commun., 2022, 58, 5399 DOI: 10.1039/D2CC01372J

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Chemical Communications

Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA†

Abstract

Supplementary files

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Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA

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