A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells

Ya Zhang; Huishan Li; Hailing Mai; Dong Luo; Xinpei Ji; Zhengting Liu; Shiyong Peng; Xuetao Xu; Yinghui Zhang; Rongfeng Lan; Hongguang Li

doi:10.1039/D2CC01211A

A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells†

Ya Zhang,‡^a Huishan Li,‡^a Hailing Mai,^a Dong Luo,^a Xinpei Ji,^a Zhengting Liu,^a Shiyong Peng,

^a Xuetao Xu,

^a Yinghui Zhang,^ab Rongfeng Lan

*^c and Hongguang Li

*^ab

Author affiliations

* Corresponding authors

^a Guangdong Provincial Key Laboratory of, Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen 529020, China
E-mail: wyuchemlihg@126.com

^b South China Institute of Large Animal Models for Biomedicine, Wuyi University, Jiangmen 529020, China

^c College of Basic medical sciences, Shenzhen University Health Science Center, Shenzhen 518060, China
E-mail: lan@szu.edu.cn

Abstract

In this study, we designed and tested fluorescent probe zy-2 for specific and responsive imaging of pyruvate kinase M2 (PKM2), which can be excited by 419 nm light. A 17-fold enhancement in the responsive emission upon zy-2's binding to PKM2 was observed, imaging of which was successfully recorded in a time- and concentration-dependent manner in PKM2-positive cells. Thus, we obtained a responsive fluorescent probe for the specific and sensitive detection of PKM2, which is innovative in design and applicable to the detection of cancer cells.

Chemical Communications

A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells†

Abstract

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A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells

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