Issue 4, 2022
From the journal:

Chemical Communications

A DNAzyme-based normalized fluorescence strategy for direct quantification of endogenous zinc in living cells†

Xuanhao Zhang,a   Zhi-Ling Song,*a   Qiqi Chao,a   Qian Li,a   Rongmei Kong, ORCID logo b   Gao-Chao Fan ORCID logo a  and  Xiliang Luo ORCID logo *a  

* Corresponding authors

a Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
E-mail: zhilingsong@qust.edu.cn, xiliangluo@qust.edu.cn

b School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, People's Republic of China

Abstract

Taking the maximum fluorescence of an identical fluorophore as a reference, a DNAzyme-based normalized strategy is developed to unify the output signals under external interferences. This makes it possible to directly quantify endogenous zinc in living cells by in situ fluorescence imaging, implying promising potential in fundamental study and early disease diagnosis.

Graphical abstract: A DNAzyme-based normalized fluorescence strategy for direct quantification of endogenous zinc in living cells

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Article information

DOI
https://doi.org/10.1039/D1CC06471A
Article type
Communication
Submitted
17 Nov 2021
Accepted
09 Dec 2021
First published
09 Dec 2021

Chem. Commun., 2022,58, 577-580

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A DNAzyme-based normalized fluorescence strategy for direct quantification of endogenous zinc in living cells

X. Zhang, Z. Song, Q. Chao, Q. Li, R. Kong, G. Fan and X. Luo, Chem. Commun., 2022, 58, 577 DOI: 10.1039/D1CC06471A

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