Issue 16, 2022

CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

Abstract

We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL−1, which is quite superior to those of alternative approaches.

Graphical abstract: CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

Supplementary files

Article information

Article type
Communication
Submitted
26 Oct 2021
Accepted
29 Nov 2021
First published
20 Dec 2021

Chem. Commun., 2022,58, 2654-2657

CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

H. Kim, S. Lee, J. Lee and H. G. Park, Chem. Commun., 2022, 58, 2654 DOI: 10.1039/D1CC06026K

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