Issue 12, 2022
From the journal:

Chemical Communications

Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR–Cas12a†

Heyjin Son,‡a   Jaeil Park,‡ab   You Hee Choi,ac   Youngri Jung,d   Joong-Wook Lee,b   Sangsu Baed  and  Sanghwa Lee ORCID logo *a  

* Corresponding authors

a Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea
E-mail: sanglee@gist.ac.kr

b Department of Physics and Optoelectronics Convergence Research Center, Chonnam National University, Gwangju 61186, Republic of Korea

c Ministry of Food and Drug Safety (MFDS), Cheongju 28159, Republic of Korea

d Department of Chemistry, Hanyang University, Seoul 04763, Republic of Korea

Abstract

CRISPR–Cas12a has been widely used in genome editing and nucleic acid detection. In both of these applications, Cas12a cleaves target DNA in a divalent metal ion-dependent manner. However, when and how metal ions contribute to the cleavage reaction is unclear. Here, using a single-molecule FRET assay, we reveal that these metal ions are necessary for stabilising cleavage-competent conformations and that they are easily exchangeable, suggesting that they are dynamically coordinated.

Graphical abstract: Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR–Cas12a

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Article information

DOI
https://doi.org/10.1039/D1CC04446J
Article type
Communication
Submitted
12 Aug 2021
Accepted
13 Jan 2022
First published
14 Jan 2022
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2022,58, 1978-1981

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Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR–Cas12a

H. Son, J. Park, Y. H. Choi, Y. Jung, J. Lee, S. Bae and S. Lee, Chem. Commun., 2022, 58, 1978 DOI: 10.1039/D1CC04446J

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