A signal-on fluorescent biosensor for mercury detection based on a cleavable phosphorothioate RNA fluorescent probe and metal–organic frameworks

Abstract

Mercury contamination is a major environmental concern. In this work, we used a cleavable phosphorothioate (PS) fluorescence probe quenched by UiO-66-NH₂ to develop a “signal-on” fluorescent biosensor for Hg²⁺ detection. The probe was bound to UiO-66-NH₂ through π–π stacking and hydrogen bonding, thereby extinguishing the fluorescence of the FAM-labelled probe. The PS site was cleaved in the presence of Hg²⁺, releasing the FAM group and significantly enhancing the fluorescence signal. The intensity of the fluorescence linearly rose as the Hg²⁺ concentration increased in the range of 1–100 nM (R² = 0.994), and the limit of detection was 0.118 nM (S/N = 3). This biosensor demonstrated high selectivity for Hg²⁺ and was effectively applied to quantification of Hg²⁺ in various water samples with acceptable recovery rates. These results suggest that this practical, straightforward technology is a good option for monitoring mercury ions in the environment.