Application of ComplexGAPI for the green assessment of a deep eutectic solvent-based ferrofluid assisted liquid–liquid microextraction method for detection of dimethyl phthalate in beverage samples

Abstract

In the present study, a novel ferrofluid was prepared by combining a menthol/thymol deep eutectic solvent with magnetic nanoparticles (Fe₃O₄@OA). This composite was first applied in vortex-assisted liquid–liquid microextraction (VA-LLME), followed by high performance liquid chromatography with ultraviolet detection (HPLC-UV) for the determination of dimethyl phthalate (DMP) residues in beverages. The synthesized deep eutectic solvent-based ferrofluid (DES-FF) was characterized by using Fourier transform infrared spectrometry (FTIR), vibrating sample magnetometry (VSM), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). Furthermore, the type of carrier, pH of the sample solution, ferrofluid volume, salt amount, vortex time, type and volume of elution solvents and desorption time were statistically optimized for high extraction efficiencies. Under the optimal extraction conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 0.008 μg mL⁻¹ and 0.03 μg mL⁻¹, respectively. Moreover, the mean recoveries for DMP ranged from 85.2% to 99.5%, and intra- and inter-day precisions were less than 5.5% and 7.8%, respectively. The proposed method was successfully applied to the analysis of dimethyl phthalate in real samples, making it a promising analysis technique for beverage samples. The greenness of the entire procedure of our proposed method was assessed by comparing it with other reported methods using ComplexGAPI (Complex Green Analytical Procedure Index). The results show that our proposed method has a better greenness than other reported methods.