Issue 11, 2022

Fluorescence immunoassay for the targeted determination of trace Listeria monocytogenes based on immunomagnetic separation and CdZnTe quantum dot indication

Abstract

Infections from invasive Listeria monocytogenes (L. monocytogenes) frequently occur in food and can cause high morbidity and death. Thus, the sensitive, specific, and rapid detection of L. monocytogenes is critical for ensuring food safety and public health. Herein, a fluorescence immunoassay for trace L. monocytogenes detection was designed based on guinea pig antibody-functionalized magnetic nanoparticles (Fe3O4 NPs/pAb1) and rabbit antibody-anchored CdZnTe quantum dots (CdZnTe QDs/pAb2). Because of the antibody-directed magnetic separation and long-wave fluorescent emission for CdZnTe QD indication, the constructed immunoassay strategy presented excellent anti-interference performance toward a biological matrix. The immunosensor exhibited a wide detection range of 1 to 109 CFU mL−1 for L. monocytogenes and a low limit of detection (LOD) of 1 CFU mL−1, achieving an exceptionally sensitive detection of trace L. monocytogenes. Meanwhile, the immunosensor showed good specificity and had a short time-consumption of 60 min to realize the accurate determination of trace Listeria monocytogenes in spiked tap water and pasteurized milk samples.

Graphical abstract: Fluorescence immunoassay for the targeted determination of trace Listeria monocytogenes based on immunomagnetic separation and CdZnTe quantum dot indication

Supplementary files

Article information

Article type
Paper
Submitted
11 Dec 2021
Accepted
11 Feb 2022
First published
11 Feb 2022

Anal. Methods, 2022,14, 1124-1133

Fluorescence immunoassay for the targeted determination of trace Listeria monocytogenes based on immunomagnetic separation and CdZnTe quantum dot indication

S. Liang, L. Ji, Y. Zhong, T. Wang, H. Yang, Q. Li, X. Li and S. Zhao, Anal. Methods, 2022, 14, 1124 DOI: 10.1039/D1AY02106K

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