Issue 4, 2022

Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

Abstract

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

Graphical abstract: Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

Supplementary files

Article information

Article type
Communication
Submitted
15 Aug 2021
Accepted
17 Dec 2021
First published
20 Dec 2021

Anal. Methods, 2022,14, 378-382

Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

G. D. Suarez, D. A. Suarez, Y. Y. Kiu Tang, J. Zhang, J. Li, S. Nagl and P. P. Cheung, Anal. Methods, 2022, 14, 378 DOI: 10.1039/D1AY01395E

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