Cell-membrane-targeted near-infrared fluorescent probe for detecting extracellular ATP

Abstract

Extracellular adenosine triphosphate (ATP) as a signal molecule plays a key role in tumor progression and metastasis. Therefore, the development of a fluorescent probe to detect extracellular ATP is crucial for tumor treatment. However, small-molecule fluorescent probes have better advantages than biological probes, such as low price, easy modification, and optical tunability, but still remain highly challenging and rarely explored in extracellular ATP detection. Here, a near-infrared small molecule fluorescent probe (NIR-P) with hydrophobic alkyl chains and hydrophilic macrocyclic polyamines was prepared for the detection of extracellular ATP. The NIR-P exhibited enhanced fluorescence upon binding to ATP by electrostatic interaction and π–π interaction between phosphates and macrocyclic polyamines, adenines and benzene rings with a limit of detection (LOD) of 21 nM. In addition, with similarity and intermiscibility to the cell membrane, the NIR-P can specifically target cell membranes and image extracellular ATP. This work provides a cell-membrane-targeted fluorescent probe used for extracellular ATP detection.