Issue 5, 2022

Optimization of measurement mode and sample processing for FTIR microspectroscopy in skin cancer research

Abstract

The use of Fourier Transform Infrared (FTIR) microspectroscopy to study cancerous cells and tissues has gained popularity due to its ability to provide spatially resolved information at the molecular level. Transmission and transflection are the commonly used measurement modes for FTIR microspectroscopy, and the tissue samples measured in these modes are often paraffinized or deparaffinized. Previous studies have shown that variability in the spectra acquired using different measurement modes and sample processing methods affect the result of the analysis. However, there is no protocol that standardizes the mode of measurement and sample processing method to achieve the best classification result. This study compares the spectra of primary (IPC-298) and metastatic (SK-MEL-30) melanoma cell lines acquired in both transmission and transflection modes using paraffinized and deparaffinized samples to determine the optimal combination for accurate classification. Significant differences were observed in the spectra of the same cell line measured in different modes and with or without deparaffinization. The PLS-DA model built for the classification of two cell lines showed high accuracy in each case, suggesting that both modes and sample processing alternatives are suitable for differentiating cultured cell samples using supervised multivariate analysis. The biochemical information contained in the cells capable of discriminating two melanoma cell lines is present regardless of mode or sample type used. However, the paraffinized samples measured in transflection mode provided the best classification.

Graphical abstract: Optimization of measurement mode and sample processing for FTIR microspectroscopy in skin cancer research

Article information

Article type
Paper
Submitted
03 Nov 2021
Accepted
28 Jan 2022
First published
31 Jan 2022
This article is Open Access
Creative Commons BY license

Analyst, 2022,147, 851-861

Optimization of measurement mode and sample processing for FTIR microspectroscopy in skin cancer research

B. R. Shakya, H. Teppo and L. Rieppo, Analyst, 2022, 147, 851 DOI: 10.1039/D1AN01999F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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