Jump to main content
Jump to site search

Issue 18, 2021
Previous Article Next Article

Influence of dityrosine nanotubes on the expression of dopamine and differentiation in neural cells

Author affiliations

Abstract

In this study, we report the synthesis of self-assembled dityrosine nanotubes as a biologically functional scaffold and their interactions with neural cells. Quantum chemical methods were used to determine the forces involved in the self-assembly process. The physicochemical properties of the nanostructures relevant to their potential as bioactive scaffolds were characterized. The morphology, secondary structure, crystallinity, mechanical properties, and thermal characteristics of YY nanotubes were analyzed. The influence of these nanotubes as scaffolds for neural cells was studied in vitro to understand their effects on cell proliferation, morphology, and gene expression. The scanning electron microscopy and fluorescence confocal microscopy demonstrated the feasibility of nanotube scaffolds for enhanced adhesion to rat and human neural cells (PC12 and SH-SY5Y). Preliminary ELISA and qPCR analyses demonstrate the upregulation of dopamine synthesis and genes involved in dopamine expression and differentiation. The expression levels of DβH, AADC, VMAT2 and MAOA in SH-SY5Y cells cultured on the nanotube scaffolds for 7 days were elevated in comparison to the control cells.

Graphical abstract: Influence of dityrosine nanotubes on the expression of dopamine and differentiation in neural cells

Back to tab navigation

Supplementary files

Article information


Submitted
16 Nov 2020
Accepted
09 Apr 2021
First published
30 Apr 2021

J. Mater. Chem. B, 2021,9, 3900-3911
Article type
Paper

Influence of dityrosine nanotubes on the expression of dopamine and differentiation in neural cells

P. Macha, M. L. Mayes, B. Rey. B. Visayas, V. Soni, V. R. Sammeta and M. C. Vasudev, J. Mater. Chem. B, 2021, 9, 3900
DOI: 10.1039/D0TB02680H

Social activity

Search articles by author

Spotlight

Advertisements