Synthesis and characterization of electroconductive hydrogels based on oxidized alginate and polypyrrole-grafted gelatin as tissue scaffolds

Abstract

Electroconductive biocompatible hydrogels with tunable properties have extensively been taken into account in tissue engineering applications due to their potential to provide suitable microenvironmental responses for the cells. In the present study, novel electroconductive hydrogels are designed and synthesized by reacting oxidized alginate with polypyrrole-grafted gelatin copolymer (PPy-g-gelatin) via formation of a Schiff-base linkage. The influence of the composition and the concentration of the components on the compressive modulus and functional performance of the hydrogels is investigated. The conductivity of the hydrogels measured by a two-probe method increased by increasing the level of polypyrrole-grafted gelatin, and a conductivity of 0.7753 S m⁻¹ was exhibited by the hydrogel composed of 8% w/v polypyrrole-grafted gelatin (oxidized alginate:gelatin:polypyrrole-grafted gelatin; 30 : 35 : 35% v/v). The hydrogel compressive modulus was shown to be enhanced by increasing the total concentration of hydrogel. The characteristic features of the prepared hydrogels, including swelling ratio, volume fraction, cross-link density, and mesh size, are also studied and analyzed. Besides, the conductive hydrogels have a smaller mesh size and higher cross-link density than the non-conductive hydrogels. However, the hydrogels with high cross-link density, small mesh size, and large pore size presented higher electroconductivity as a result of easier movement of the ions throughout the hydrogel. These conductive hydrogels exhibited electrical conductivity and biodegradability with cell viability, implying potential as scaffolds for tissue engineering.