Issue 29, 2021

Harnessing α-l-fucosidase for in vivo cellular senescence imaging


Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of α-L-fucosidase (α-fuc) as a biomarker for cellular senescence and the development of an α-fuc-responsive aggregation induced emission (AIE) probe, termed QM-NHαfuc designed to complement more conventional probes based on β-galactosidase (β-gal). Using QM-NHαfuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively. QM-NHαfuc also proved capable of identifying senescent cells lacking β-gal expression. The non-invasive real-time senescence tracking provided by QM-NHαfuc was validated in an in vivo senescence model. The results presented in this study lead us to suggest that the QM-NHαfuc could emerge as a useful tool for investigating senescence processes in biological systems.

Graphical abstract: Harnessing α-l-fucosidase for in vivo cellular senescence imaging

Supplementary files

Article information

Article type
Edge Article
23 Apr 2021
24 Jun 2021
First published
25 Jun 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2021,12, 10054-10062

Harnessing α-L-fucosidase for in vivo cellular senescence imaging

S. Koo, M. Won, H. Li, W. Y. Kim, M. Li, C. Yan, A. Sharma, Z. Guo, W. Zhu, J. L. Sessler, J. Y. Lee and J. S. Kim, Chem. Sci., 2021, 12, 10054 DOI: 10.1039/D1SC02259H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity