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The Ras Dimer Structure

Abstract

Oncogenic mutated Ras is a key player in cancer, but despite intense and expensive approaches its catalytic center seems undruggable. The Ras dimer interface is a possible alternative drug target. Dimerization at the membrane affects cell growth signal transduction. In vivo studies indicate that preventing dimerization of oncogenic mutated Ras inhibits uncontrolled cell growth. Conventional computational drug-screening approaches require a precise atomic dimer model as input to successfully access drug candidates. However, the proposed dimer structural models are controversial. Here, we provide a clear-cut experimentally validated N-Ras dimer structural model. We incorporated unnatural amino acids into Ras to enable the binding of labels at multiple positions via click chemistry. This labeling allowed for the determination of multiple distances of the membrane-bound Ras-dimer measured by fluorescence and electron paramagnetic resonance spectroscopy. In combination with protein-protein docking and biomolecular simulations we identified key residues for dimerization. Site‑directed mutations of these residues prevent dimer formation in our experiments, proving our dimer model to be correct. The presented dimer structure enables now computational drug-screening studies exploiting the Ras dimer interface as alternative drug target.

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Supplementary files

Article information


Submitted
17 Feb 2021
Accepted
29 Apr 2021
First published
04 May 2021

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2021, Accepted Manuscript
Article type
Edge Article

The Ras Dimer Structure

T. Rudack, C. Kötting, C. Teuber, M. Scherlo, J. Güldenhaupt, J. Schartner, M. Lübben, J. P. Klare and K. Gerwert, Chem. Sci., 2021, Accepted Manuscript , DOI: 10.1039/D1SC00957E

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