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Issue 35, 2021

Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

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Abstract

A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation of N-acetyl-D-mannosamine with sodium pyruvate to N-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated in c-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use of c-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.

Graphical abstract: Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

Supplementary files

Article information


Submitted
04 Jun 2021
Accepted
14 Jun 2021
First published
21 Jun 2021

This article is Open Access

RSC Adv., 2021,11, 21857-21861
Article type
Paper

Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

M. T. de Martino, F. Tonin, V. R. L. J. Bloemendal, U. Hanefeld, F. P. J. T. Rutjes and J. C. M. van Hest, RSC Adv., 2021, 11, 21857 DOI: 10.1039/D1RA04332C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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