Sensitive and selective determination of tetracycline in milk based on sulfur quantum dot probes

Abstract

A novel fluorescent probe based on sulfur quantum dots (SQDs) was fabricated for sensitive and selective detection of tetracycline (TC) in milk samples. The blue emitting SQDs were synthesized via a top–down method with assistance of H₂O₂. The synthesized SQDs showed excellent monodispersity, water solubility and fluorescence stability, with a quantum yield (QY) of 6.30%. Furthermore, the blue fluorescence of the obtained SQDs could be effectively quenched in the presence of TC through the static quenching effect (SQE) and inner filter effect (IFE) between TC and SQDs. Under the optimum conditions, a rapid detection of TC could be accomplished within 1 min and a wide linear range could be obtained from 0.1 to 50.0 μM with a limit of detection (LOD) of 28.0 nM at a signal-to-noise ratio of 3. Finally, the SQD-based fluorescent probe was successfully applied for TC determination in milk samples with satisfactory recovery and good relative standard deviation (RSD). These results indicate that the SQD-based fluorescent probe shows great potential in practical analysis of TC in real samples with high rapidity, selectivity, and sensitivity.