Preparative separation of gallic acid from Fallopia aubertii using middle-pressure chromatogram isolated gel coupled with reversed-phase chromatography with hydrophilic groups
Fallopia aubertii (L. Henry) Holub is a traditional Tibetan medicine to treat gout, but the research on its chemical composition is limited, probably due to the complex purification process. In this study, the chemical constituents of F. aubertii were isolated and identified by medium pressure chromatography and high-pressure preparative liquid chromatography. The general separation process was as follows: first, the extraction part of ethyl acetate collected seven components according to the chromatographic peaks of the sample through the medium pressure chromatographic separation system. Next, the separation effects of Fr2 on five different preparation columns were compared. A reversed-phase chromatographic column with hydrophilic groups was selected for separation. Finally, the principal component compound (gallic acid) in Fr2 was isolated and identified. The results show that the combination of medium pressure chromatographic separation gel column and reversed phase chromatographic column with hydrophilic groups is an effective separation method for the separation of gallic acid from F. aubertii.