Highly sensitive non-enzymatic hydrogen peroxide monitoring platform based on nanoporous gold via a modified solid-phase reaction method

Abstract

In this work, nanoporous gold (NPG) fabricated using a modified solid-phase reaction method was developed as an electrocatalyst for the nonenzymatic detection of hydrogen peroxide (H₂O₂). The NPG morphology and structure were characterized by scanning electron microscopy and high-resolution transmission electron microscopy. The fabricated NPG exhibited a nanoporous framework with numerous structural defects. The NPG-based amperometric H₂O₂ sensor had a good selectivity, reproducibility, and low detection limit (0.3 μM) under near physiological conditions (pH = 7.4). The sensitivities of this sensor over concentration ranges of 0.002–5 mM and 5–37.5 mM were 159 and 64 μA mM⁻¹ cm⁻², respectively. These results indicate that the developed NPG is a promising material for the electrochemical sensing of H₂O₂.