Methane production from wheat straw pretreated with CaO2/cellulase

Abstract

There are various lignocellulosic biomass pretreatments that act as attractive strategies to improve anaerobic digestion for methane (CH₄) generation. This study proposes an effective technique to obtain more CH₄ via the hydrothermal coupled calcium peroxide (CaO₂) co-cellulase pretreatment of lignocellulosic biomass. The total organic carbon in the hydrolysate of samples treated with 6% CaO₂ and 15 mg enzyme per g-cellulose was 7330 mg L⁻¹, which represented an increase of 92.39% over the total organic carbon value of samples hydrolyzed with the enzyme alone. The promotion of the anaerobic digestion of wheat straw followed this order: hydrothermal coupled CaO₂ co-cellulase pretreatment > hydrothermal coupled CaO₂ pretreatment > enzymatic pretreatment alone > control group. The sample treated with 6% CaO₂ and 15 mg enzyme per g-cellulose gave the highest CH₄ production with a CH₄ yield of 214 mL g⁻¹ total solids, which represented an increase of 64.81% compared to the control group. The CH₄ yield decreased slightly when the amount of added cellulase exceeded 15 mg enzyme per g-cellulose.