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Issue 25, 2021, Issue in Progress
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Demonstration of pH-controlled DNA–surfactant manipulation for biomolecules

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Abstract

The understanding of DNA–surfactant interactions is important for fundamental physical biology and developing biomedical applications. In the present study, we demonstrated a DNA–surfactant nano-machine model by modulating the compaction of DNA in dodecyldimethylamine oxide (DDAO) solutions. By controlling DDAO concentration and pH of solution, we are able to adjust the compacting force of DNA so as to pull biomolecular subunits connected to it. The pulling force of the machine depends on DDAO concentration and pH of solution, ranging from near zero to about 4.6 pN for 10 mM DDAO concentration at pH = 4. The response time of the machine is about 3 minutes for contracting and 2 minutes for releasing in 5 mM DDAO solution. We found that DDAO has no significant influence on DNA under basic conditions, but compacts DNA under acidic conditions, which is enhanced with decreasing pH of solution. Meanwhile, we found the accompanying charge inversion of DNA in the process of DNA compaction by DDAO.

Graphical abstract: Demonstration of pH-controlled DNA–surfactant manipulation for biomolecules

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Article information


Submitted
22 Feb 2021
Accepted
18 Apr 2021
First published
22 Apr 2021

This article is Open Access

RSC Adv., 2021,11, 15099-15105
Article type
Paper

Demonstration of pH-controlled DNA–surfactant manipulation for biomolecules

N. Li, Z. Liao, S. He, X. Chen, S. Huang, Y. Wang and G. Yang, RSC Adv., 2021, 11, 15099
DOI: 10.1039/D1RA01420J

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