Issue 20, 2021, Issue in Progress

Microwave-assisted xylanase reaction: impact in the production of prebiotic xylooligosaccharides

Abstract

The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. In this work, thermostable alkali-tolerant xylanase (BhXyn10A) from Bacillus halodurans S7 has been used to produce XOS under conventional convective heat transfer and microwave radiation. The microwave-assisted reaction markedly decreases the xylose content in the hydrolysates and significantly increases the yield of XOS, compared to conventional heating. Molecular dynamics simulations of BhXyn10A have shown increased fluctuations of the amino acids of the aglycone subsites suggesting that these subsites can determine the production of xylose. Thus, microwave heating could affect the amino acid fluctuations in the aglycone subsites reducing the xylose formation. These findings open up new avenues in enzyme technology for the production of XOS.

Graphical abstract: Microwave-assisted xylanase reaction: impact in the production of prebiotic xylooligosaccharides

Supplementary files

Article information

Article type
Paper
Submitted
18 Jan 2021
Accepted
17 Mar 2021
First published
23 Mar 2021
This article is Open Access
Creative Commons BY license

RSC Adv., 2021,11, 11882-11888

Microwave-assisted xylanase reaction: impact in the production of prebiotic xylooligosaccharides

H. Mobarec, R. Villagomez, E. Nordberg Karlsson and J. A. Linares-Pastén, RSC Adv., 2021, 11, 11882 DOI: 10.1039/D1RA00449B

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