Fungal remediation of Cd(ii) from wastewater using immobilization techniques

Abstract

The pollution of wastewater by heavy metal ions is hazardous to the environment and human health. Cd(II) has been recognized as one of the heavy metals that causes severe toxic effects. The present study is aimed at removing Cd(II) from wastewater using fungal biomass either immobilized on loofa sponges or in Ca-alginate beads. Two fungal species were isolated from pools of Cd(II)-polluted wastewater obtained from some Egyptian industrial plants, and using internal transcribed spacer (ITS) primers, they were molecularly identified as Penicillium chrysogenum and Cephalotheca foveolata with accession numbers MT664773 and MT664745, respectively. The sorbents used in this study were heat-inactivated mycelia of P. chrysogenum (PEN), heat-inactivated mycelia of C. foveolata (CEP), P. chrysogenum immobilized on loofa sponge (PEN-ILS), C. foveolata immobilized on loofa sponge (CEP-ILS), P. chrysogenum immobilized in Ca-alginate beads (PEN-IA), and C. foveolata immobilized in Ca-alginate beads (CEP-IA). The effects of pH, contact time, initial Cd(II) concentration, and interfering ions on Cd(II) removal from aqueous solution were tested. Maximum Cd(II) sorption capacity was obtained at pH 7.0, with thirty minutes contact time and 0.5 mol l⁻¹ initial Cd(II) concentration for all sorbents used. However, Ca²⁺ displayed synergistic interference with Cd(II) that was greater than that from Na⁺ and K⁺, with decreasing sorption capacity for all sorbents. Optimum conditions were applied to real wastewater samples collected from two Egyptian industrial plants. All sorbents had the ability to remove Cd(II) from wastewater samples, and enhanced removal occurred when fungal cells were immobilized as compared to free cells.