Issue 23, 2021

Rapid detection of thioredoxin reductase with a fluorescent probe via a Tag-Sec method

Abstract

Thioredoxin reductase (TrxR), which plays a key role in cellular regulations including cell growth, redox signaling pathways and differentiation, belongs to the selenocysteine-containing protein family. Numerous human diseases are associated with thioredoxin reductase/selenocysteine deficiency or overdose. Thus, there is an urgent need to develop new tools for thioredoxin reductase detection. Small molecule fluorescent probes, which have been studied for decades, are powerful chemical tools for biomolecule detection and diagnosis within a bio-system. However, there still remains a challenge in finding a specific target site when designing a new probe. In this work, we presented a Tag-Sec fluorescent probe that could detect both thioredoxin reductase and selenocysteine with high sensitivity, selectivity, broad pH tolerance and good water solubility. We hope that the concept described in this work could serve as a useful guide to design probes for detecting Sec-containing proteins and clinical diagnosis.

Graphical abstract: Rapid detection of thioredoxin reductase with a fluorescent probe via a Tag-Sec method

Supplementary files

Article information

Article type
Research Article
Submitted
10 Sep 2021
Accepted
20 Oct 2021
First published
20 Oct 2021

Mater. Chem. Front., 2021,5, 8108-8117

Rapid detection of thioredoxin reductase with a fluorescent probe via a Tag-Sec method

J. Ma, Y. Xu, W. Zhao, B. Wang, C. Zhang and Z. Zhang, Mater. Chem. Front., 2021, 5, 8108 DOI: 10.1039/D1QM01254A

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