Issue 46, 2021

Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

Abstract

A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase.

Graphical abstract: Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

Supplementary files

Article information

Article type
Paper
Submitted
17 Sep 2021
Accepted
07 Nov 2021
First published
09 Nov 2021

Polym. Chem., 2021,12, 6755-6760

Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

E. S. Garcia, T. M. Xiong, A. Lifschitz and S. C. Zimmerman, Polym. Chem., 2021, 12, 6755 DOI: 10.1039/D1PY01255J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements