Polymerase-mediated synthesis of p-vinylaniline-coupled fluorescent DNA for the sensing of nucleolin protein–c-myc G-quadruplex interactions

Abstract

In this paper we report the synthesis of two deoxyuridine derivatives (dU_CN2, dU_Py)—featuring p-vinylaniline-based fluorophores linked through a propargyl unit at the 5′ position—that function as molecular rotors. This probing system proved to be useful for the sensing of gene regulation arising from interactions between this G-quadruplex and nucleolin.