Issue 15, 2021

Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe

Abstract

Visualization of sub-cellular organelles allows the determination of various cellular processes and the underlying mechanisms. Herein, we report a fluorescent probe, bearing push–pull substituents emitting at 600 nm and its application in cellular imaging. The probe shows dual imaging of mitochondria and nucleoli and maps mitochondrial viscosity in live cells under various physiological variations and show minimum cytotoxicity. Nucleolar staining is confirmed by RNAase digestion.

Graphical abstract: Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe

Supplementary files

Article information

Article type
Paper
Submitted
28 Nov 2020
Accepted
22 Jan 2021
First published
27 Jan 2021

Org. Biomol. Chem., 2021,19, 3389-3395

Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe

T. Mukherjee, V. Soppina, R. Ludovic, Y. Mély, A. S. Klymchenko, M. Collot and S. Kanvah, Org. Biomol. Chem., 2021, 19, 3389 DOI: 10.1039/D0OB02378G

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