A near-infrared fluorescent probe that can image endogenous hydrogen polysulfides in vivo in tumour-bearing mice

Abstract

Hydrogen polysulfides (H₂S_n, n > 1), which are important reactive sulfur species, play crucial roles in H₂S-related bioactivities, including antioxidation, cytoprotection, activation of ion channels, transcription factor functions and tumour suppression. Monitoring H₂S_nin vivo is of significant interest for exploring the physiological roles of H₂S_n and the exact mechanisms of H₂S_n-related diseases. Herein, we conceive a novel near-infrared fluorescent probe, NIR-CPS, that is used to detect H₂S_n in living cells and in vivo. With the advantages of high sensitivity, good selectivity and a remarkably large Stokes shift (100 nm), NIR-CPS was successfully applied in visualizing H₂S_n in living cells and mice. More importantly, NIR-CPS monitored H₂S_n stimulated by lipopolysaccharide in tumour-bearing mice. These results demonstrate that the NIR-CPS probe is a potentially powerful tool for the detection of H₂S_nin vivo, thus providing a valuable approach in H₂S_n-related medical research.