Rapid and simultaneous detection of heart-type fatty acid binding protein and cardiac troponin using a lateral flow assay based on metal organic framework@CdTe nanoparticles

Abstract

Herein, a zirconium metal organic framework (ZrMOF) based lateral flow assay (LFA) is described for the qualitative analysis of early acute myocardial infarction (AMI) biomarkers including heart-type fatty acid binding protein (h-FABP) and cardiac troponin (cTnT). ZrMOF@CdTe nanoparticles (NPs) are synthesized by a simple hydrothermal method. By changing the hydrothermal reaction time, ZrMOF@CdTe NPs with different fluorescent colours can be obtained. They can emit different colours of fluorescence under the excitation of a single wavelength, which makes them suitable for multiplexed lateral flow assays (mLFA). The visual limit of detection of the ZrMOF@CdTe-based LFA for the h-FABP antigen is 1 μg L⁻¹. The analysis can be completed within 8 min and has the advantages of being easy to operate and visual detection. A serum sample collected from patients is successfully detected using this LFA. This assay is widely applicable and simple, has strong anti-interference ability, and is cost-effective and can provide basic information for later treatment.