Issue 7, 2021

Visualization of molecular binding sites at the nanoscale in the lift-up mode by amplitude-modulation atomic force microscopy

Abstract

We report a new approach to visualize the local distribution of molecular recognition sites with nanoscale resolution by amplitude-modulation atomic force microscopy. By integrating chemical modification of probes, photothermal excitation to drive a cantilever, and lift-up scanning over surface topography, we successfully visualized binding sites provided by streptavidin on a solid surface for biotin attached on an AFM probe. The optimization of measurement conditions was discussed in detail, and the application of the technique was verified with a different ligand–receptor system.

Graphical abstract: Visualization of molecular binding sites at the nanoscale in the lift-up mode by amplitude-modulation atomic force microscopy

Supplementary files

Article information

Article type
Paper
Submitted
25 Aug 2020
Accepted
26 Jan 2021
First published
26 Jan 2021

Nanoscale, 2021,13, 4213-4220

Visualization of molecular binding sites at the nanoscale in the lift-up mode by amplitude-modulation atomic force microscopy

T. Maekawa, T. Nyu, E. A. Q. Mondarte, H. Tahara, K. Suthiwanich and T. Hayashi, Nanoscale, 2021, 13, 4213 DOI: 10.1039/D0NR06125E

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