Visible light driven selective NADH regeneration using a system of water-soluble zinc porphyrin and homogeneous polymer-dispersed rhodium nanoparticles

Abstract

We discovered the catalytic activity of Rh nanoparticles dispersed by polyvinylpyrrolidone (Rh–PVP) for NADH regeneration. The selective reduction of NAD⁺ to enzyme active NADH has successfully been achieved in the presence of an electron donor, ZnTPPS as a photosensitizer, and Rh–PVP. Only 1,4-NADH was produced as the reduction product of NAD⁺, which was validated by an enzymatic assay and HPLC.