Issue 20, 2021

Ultrasound-assisted enzymatic extraction of Corni Fructus alpha-glucosidase inhibitors improves insulin resistance in HepG2 cells

Abstract

Corni Fructus (CF) is a traditional medicine and beneficial food with multifaceted protective effects against diabetes and its complications. Since alpha-glucosidase inhibitors (GIs) are promising first-choice oral antihyperglycemic drugs for diabetes, we examined whether GIs from CF (GICF) are useful for diabetes treatment. Therefore, GICF was extracted by ultrasound-assisted enzymatic extraction (UAEE) that is optimized by a three-level, four-factor Box–Behnken design and determined by ultra-performance liquid chromatography. Compared to 36.31 mg g−1 without enzyme treatment, the GICF yield increased to 70.44 mg g−1via UAEE under optimum conditions (0.5% compound enzyme extracted in 23 min at 46 °C and pH 4.8). The activity (91.99%) of GICF was as predicted (93.28%). When GICF was used in an insulin-resistant HepG2 cell model, it significantly ameliorated the glucose metabolism in a dose-dependent manner. Our findings indicate that UAEE may be an innovative method for functional food extraction and a potential strategy for high-quality food ingredient (such as GI) production with high efficiency and productivity.

Graphical abstract: Ultrasound-assisted enzymatic extraction of Corni Fructus alpha-glucosidase inhibitors improves insulin resistance in HepG2 cells

Supplementary files

Article information

Article type
Paper
Submitted
02 Apr 2021
Accepted
03 Aug 2021
First published
04 Aug 2021

Food Funct., 2021,12, 9808-9819

Ultrasound-assisted enzymatic extraction of Corni Fructus alpha-glucosidase inhibitors improves insulin resistance in HepG2 cells

B. Han, H. Sun, X. Yang, T. Wang, C. He, K. Zhao, S. An, C. Guo, D. Niu, X. Xue and J. Kang, Food Funct., 2021, 12, 9808 DOI: 10.1039/D1FO01002F

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