Detecting Mycobacterium Tuberculosis using a nitrofuranyl calanolide–trehalose probe based on nitroreductase Rv2466c

Abstract

A new Mtb fluorescent probe, NFC-Tre-5, was reported that could label single cells of Mtb under various stress conditions via a unique fluorescence off–on feature by a Rv2466c-mediated reductive mechanism. This probe effectively facilitates the rapid and specific detection of Mtb in the host cell during infection and the detection of Mtb in sputum samples from patients.