Target-induced transcription of a light-up RNA aptamer to construct a novel method for alkaline phosphatase assay

Abstract

We herein developed a novel method for alkaline phosphatase (ALP) assay based on the target-induced transcription of a light-up RNA aptamer, consequently producing a highly enhanced fluorescence signal upon specifically binding to the corresponding dye. Using this strategy, we successfully determined the ALP activity down to 0.018 U L⁻¹ (dynamic linear range of 0.04–4 U L⁻¹) with excellent selectivity.