Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR

Yuting Jia; Jun Han; Hui Wang; Weixiang Hong; Honghong Wang; Mai Zhang; Zhengping Li

doi:10.1039/D1CC03033G

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Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR†

Yuting Jia,^a Jun Han,^b Hui Wang,

^a Weixiang Hong,^a Honghong Wang,

*^a Mai Zhang^a and Zhengping Li

*^a

Author affiliations

* Corresponding authors

^a Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing, China
E-mail: wanghh@ustb.edu.cn, lzpbd@ustb.edu.cn

^b National Textile and Leather Product Quality Supervision Testing Center, Beijing 100025, China

Abstract

A method based on mRNA-templated ligation of splice-junction anchored DNA probes followed by PCR amplification of the ligated product has been developed for multiplexed detection of mRNA splice variants with high sensitivity and specificity. The proposed assay can detect as low as 10 aM mRNA splicing variants and has been successfully applied to detect real samples.

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Article information

DOI: https://doi.org/10.1039/D1CC03033G
Article type: Communication
Submitted: 09 Jun 2021
Accepted: 25 Aug 2021
First published: 30 Aug 2021

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Chem. Commun., 2021,57, 10011-10014

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Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR

Y. Jia, J. Han, H. Wang, W. Hong, H. Wang, M. Zhang and Z. Li, Chem. Commun., 2021, 57, 10011 DOI: 10.1039/D1CC03033G

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Chemical Communications

Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR†

Abstract

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Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR

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