Revealing the signaling regulation of hydrogen peroxide to cell pyroptosis using a ratiometric fluorescent probe in living cells

Guanqing Yang; Tong Zhu; Dong Wang; Zhengjie Liu; Ruilong Zhang; Guangmei Han; Xiaohe Tian; Bianhua Liu; Ming-yong Han; Zhongping Zhang

doi:10.1039/D1CC02008K

Revealing the signaling regulation of hydrogen peroxide to cell pyroptosis using a ratiometric fluorescent probe in living cells†

Guanqing Yang,‡^a Tong Zhu,‡^b Dong Wang,^a Zhengjie Liu,^a Ruilong Zhang,

^ac Guangmei Han,*^a Xiaohe Tian,

^d Bianhua Liu,

^e Ming-yong Han

^e and Zhongping Zhang

^ac

Author affiliations

* Corresponding authors

^a Information Materials and Intelligent Sensing Laboratory of Anhui Province, School of Chemistry and Chemical Engineering and Institutes of Physical Science and Information Technology, Anhui University, Hefei, China
E-mail: gmhan@ahu.edu.cn

^b School of Life Science, Anhui University, Hefei 230601, China

^c Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University), Ministry of Education, Hefei, Anhui 230601, China

^d Huaxi MR Research Center (HMRRC), Department of Radiology, Functional and molecular imaging Key Laboratory of Sichuan Province, West China Hospital of Sichuan University, Chengdu 610041, China

^e Key Lab of Photovoltaic and Energy Conservation Materials, Institute of Solid State Physics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China

Abstract

A ratiometric fluorescent probe with a large emission shift was developed for the accurate measurement of hydrogen peroxide (H₂O₂) in sophisticated pyroptosis signaling pathways. The results reported here demonstrate that H₂O₂, as a principal member of ROS, is a critical upstream signaling molecule in regulating pyroptosis.

Chemical Communications

Revealing the signaling regulation of hydrogen peroxide to cell pyroptosis using a ratiometric fluorescent probe in living cells†

Abstract

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Revealing the signaling regulation of hydrogen peroxide to cell pyroptosis using a ratiometric fluorescent probe in living cells

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