Issue 36, 2021
From the journal:

Chemical Communications

Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes†

Lilin Feng,‡a   Liang Gao,‡a   Daniel F. Sauer, ORCID logo a   Yu Ji,a   Haiyang Cuia  and  Ulrich Schwaneberg ORCID logo *ab  

* Corresponding authors

a Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, Aachen 52074, Germany
E-mail: u.schwaneberg@biotec.rwth-aachen.de

b DWI – Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, Aachen, Germany

Abstract

We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(III)–metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.

Graphical abstract: Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

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Article information

DOI
https://doi.org/10.1039/D1CC01575C
Article type
Communication
Submitted
23 Mar 2021
Accepted
27 Mar 2021
First published
07 Apr 2021

Chem. Commun., 2021,57, 4460-4463

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Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

L. Feng, L. Gao, D. F. Sauer, Y. Ji, H. Cui and U. Schwaneberg, Chem. Commun., 2021, 57, 4460 DOI: 10.1039/D1CC01575C

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