Jump to main content
Jump to site search


Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Author affiliations

Abstract

Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.

Graphical abstract: Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Back to tab navigation

Supplementary files

Article information


Submitted
22 Feb 2021
Accepted
06 Apr 2021
First published
16 Apr 2021

Chem. Commun., 2021, Advance Article
Article type
Communication

Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

B. Krishnarjuna, T. Yamazaki, G. M. Anantharamaiah and A. Ramamoorthy, Chem. Commun., 2021, Advance Article , DOI: 10.1039/D1CC01018B

Social activity

Search articles by author

Spotlight

Advertisements