Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F-reporter

Abstract

Quantifying the isomeric species of metal complexes in solution is difficult. ¹⁹F NMR herein was used to determine the abundance of isomeric species and dynamic properties of lanthanide binding tags. The results suggest that ¹⁹F is an efficient reporter in assessing and screening paramagnetic tags suitable for protein NMR analysis.