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Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F-reporter

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Abstract

Quantifying the isomeric species of metal complexes in solution is difficult. 19F NMR herein was used to determine the abundance of isomeric species and dynamic properties of lanthanide binding tags. The results suggest that 19F is an efficient reporter in assessing and screening paramagnetic tags suitable for protein NMR analysis.

Graphical abstract: Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F-reporter

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Article information


Submitted
10 Feb 2021
Accepted
24 Mar 2021
First published
24 Mar 2021

Chem. Commun., 2021, Advance Article
Article type
Communication

Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F-reporter

J. Chen, B. Chen, B. Li, F. Yang and X. Su, Chem. Commun., 2021, Advance Article , DOI: 10.1039/D1CC00791B

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